EasyFRAP-web is a web-based application which facilitates qualitative and quantitative analysis of Fluorecence Recovery After Photobleaching (FRAP) data.

It is developed and maintained by the Cell Cycle Laboratory, University of Patras, Greece. The How to use and the FAQ categories contain useful information about how to use easyFRAP-web. Under Downloads, sample data for experimenting with easyFRAP-web, the easyFRAP manual appendix which details definitions and formulas used for analysis, and a link to the standalone version of easyFRAP can be found.

For questions, suggestions, bug-reports or feedback, please email us:

• easyfrap@upatras.gr

For more information consult these publications:

• Koulouras G, Panagopoulos A, Rapsomaniki MA, Giakoumakis NN, Taraviras S, Lygerou Z (2018). EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data. Nucleic Acids Research 46(W1):W467–W472 [Link]

• Rapsomaniki MA, Kotsantis P, Symeonidou IE, Giakoumakis NN, Taraviras S and Lygerou Z (2012). easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of Fluorescence Recovery After Photobleaching data. Bioinformatics 28(13):1800-1801 [Link]

• Giakoumakis NN, Rapsomaniki MA and Lygerou Z (2017). Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP). Methods Mol. Biol, 1563: 243-267 [Link]

• Rapsomaniki MA, Cinquemani E, Giakoumakis NN, Kotsantis P, Lygeros J, Lygerou Z (2015). Inference of protein kinetics by stochastic modeling and simulation of fluorescence recovery after photobleaching experiments. Bioinformatics 31:355-362. [Link]

The Cell Cycle Laboratory, headed by Prof. Zoi Lygerou, is studying cell cycle control and the maintenance of genome integrity. We combine functional live-cell imaging approaches to modeling and in silico analysis to study protein complexes maintaining genome stability across evolution.