EasyFRAP-web is a web-based application which facilitates qualitative and quantitative analysis of Fluorecence Recovery After Photobleaching (FRAP) data.

It is developed and maintained by the Cell Cycle Laboratory, University of Patras, Greece. The How to use and the FAQ categories contain useful information about how to use easyFRAP-web. Under Downloads, sample data for experimenting with easyFRAP-web, the easyFRAP manual appendix which details definitions and formulas used for analysis, and a link to the standalone version of easyFRAP can be found.

For questions, suggestions, bug-reports or feedback, please email us:

• easyfrap {at symbol} upatras {dot symbol} gr

For more information consult these publications:

• Koulouras G, Panagopoulos A, Rapsomaniki MA, Giakoumakis NN, Taraviras S, Lygerou Z (2018). EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data. Nucleic Acids Research 46(W1):W467–W472 

• Rapsomaniki MA, Kotsantis P, Symeonidou IE, Giakoumakis NN, Taraviras S and Lygerou Z (2012). easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of Fluorescence Recovery After Photobleaching data. Bioinformatics 28(13):1800-1801 

• Giakoumakis NN, Rapsomaniki MA and Lygerou Z (2017). Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP). Methods Mol. Biol, 1563: 243-267 

• Rapsomaniki MA, Cinquemani E, Giakoumakis NN, Kotsantis P, Lygeros J, Lygerou Z (2015). Inference of protein kinetics by stochastic modeling and simulation of fluorescence recovery after photobleaching experiments. Bioinformatics 31:355-362. 

The Cell Cycle Laboratory, headed by Prof. Zoi Lygerou, is studying cell cycle control and the maintenance of genome integrity. We combine functional live-cell imaging approaches to modeling and in silico analysis to study protein complexes maintaining genome stability across evolution.

The software is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and non-infringement. In no event shall the authors or copyright holders be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of or in connection with the software or the use or other dealings in the software. The authors make no warranties about the accuracy, reliability, completeness, or timeliness of the material, services, software, text, graphics, and links. Description of, or references to, products or publications does not imply endorsement of that product or publication. By using this site you agree to assume all risks associated with your use or transfer of any and all information contained on this site.

Like many site operators, we collect information that your browser sends whenever you visit our Site ("Log Data"). This Log Data may include information such as your computer's Internet Protocol ("IP") address, browser type, browser version, the pages of our website that you visit, the time and date of your visit, the time spent on those pages and other statistics. In addition, we may use third party services such as Google Analytics that collect, monitor and analyze website traffic, in order to track usage statistics and to identify operational problems. This information is not used to identify individuals or organizations, and is never shared with third parties. Cookies are used by the search pages in order to remember your analysis settings. Some cookies persist after you exit the browser, but they are never used for either identification or tracking purposes.

EasyFRAP-web is part of ELIXIR - Greece, the Greek Node of the distributed european infrastructure ELIXIR - Europe. The “ELIXIR-GR: Managing and Analysing Life Sciences Data (MIS: 5002780)” Project is co-financed by Greece and the European Union - European Regional Development Fund.